We received AMC-HN-8 and Hep-2 cells from Cell Bank of Chinese Academy of Sciences (Shanghai, China). The Ethics Committee in Shanghai Gongli Hospital approved the investigation.
We obtained written informed consent from all patients. No patients received chemotherapy and/or radiotherapy prior to surgery. In total, we obtained 30 paired LSCC tissues along with adjacent nontumor tissues from patients in the hospital. Therefore, the current study is aimed at revealing the regulatory mechanism concerning hsa_circ_0023305 expression in LSCC. However, the precise relationship between hsa_circ_0023305 expression and LSCC progression remains unclear. Among these circRNAs, hsa_circ_0023305 expression was increased in LSCC tissues. Our study with high-throughput sequencing identified many circRNAs with abnormal expression. The circular RNA circCORO1C promotes LSCC progression via modulation of the let-7c-5p/PBX3 axis. Previous studies have found that circRNA_100290 overexpression enhances LSCC progression via the miR-136-5p/RAP2C axis. These circRNAs regulate gene expression at the posttranscriptional or transcriptional level by binding to and inhibiting the function of microRNAs (miRNAs) and other molecules.
Emerging evidence has identified the presence of endogenous circRNAs in mammalian cells. circRNAs have a relatively jarless framework, and their expression is tissue-specific in the eukaryotic transcriptome. circRNAs primarily function as noncoding RNA molecules that are comprised of a covalently closed continuous loop without 5 -3 polarity or a poly-A tail. circRNAs are an abundant type of endogenous RNA and have recently been rediscovered and reevaluated for their essential functions in the regulation of gene expression. Recently, circular RNAs (circRNAs) have received attention regarding their correlation with human diseases, particularly cancer, suggesting a potential use for circRNAs as novel biomarkers and therapeutic targets. As such, early diagnosis and treatment are extremely indispensable for lowering laryngeal cancer mortality. Poor differentiation and lymph node metastases are associated with reduced five-year survival rate. The five-year survival rate of LSCC is greater than 90% with early treatment metastasis and recurrence are the main factors that influence LSCC prognosis. Currently, LSCC treatment primarily includes radiation therapy, surgery, and chemotherapy along with biological therapy. Laryngeal squamous cell carcinoma (LSCC) is the most general class of laryngeal cancer. Laryngeal cancer is a malignancy of the larynx that accounts for 1–5% of total malignant tumor incidence. In summary, these data propose a new mechanism by which the hsa_circ_0023305/miR-218-5p/TRPM7 network enhances LSCC progression. Additionally, we found that hsa_circ_0023305-mediated upregulation of TRPM7 inhibited miR-218-5p and contributed to LSCC migration, proliferation, and invasion. Our data demonstrated that miR-218-5p was downregulated in LSCC and that miR-218-5p upregulation repressed LSCC proliferation and invasion both in vivo and in vitro. TRPM7 regulates a nonselective cation channel and promotes cancer proliferation and metastasis. Our team validated that hsa_circ_0023305 functioned as a miR-218-5p sponge from a mechanistic perspective, targeting the melastatin-related transient receptor potential 7 (TRPM7) in LSCC cells. Knockdown of hsa_circ_0023305 significantly inhibited LSCC cell proliferation, invasion, and migration abilities. Moreover, higher hsa_circ_0023305 levels were correlated to poorer LSCC patient outcomes. Furthermore, we discovered that hsa_circ_0023305 expression level was positively correlated to tumor/node/metastasis (TNM) stage as well as lymph node metastasis in LSCC. Recent investigations using bioinformatics analysis revealed high expression of hsa_circ_0023305 in LSCC tissues compared to normal tissues. However, the circRNA mechanism in laryngeal squamous cell carcinoma (LSCC) remains elusive. Recently, circular RNAs have been shown to function as critical regulators of many human cancers.
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